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ISR-beta ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human ISR-beta.
Catalog No. ABIN5068084

Quick Overview for ISR-beta ELISA Kit (ABIN5068084)

Target

See all ISR-beta ELISA Kits
ISR-beta (Insulin Receptor beta (ISR-beta))

Reactivity

  • 3
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Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Application

ELISA

Sample Type

Cell Lysate
  • Purpose

    This IR (beta-subunit) ELISA is designed to detect and quantify levels of the insulin receptor b-subunit independent of its phosphorylation status. Both natural (heterotetrameric) and recombinant IR react in this assay. This ELISA is not cross-reactive with IGF-1R. Although this ELISA kit is developed using cells containing human insulin receptor, mouse and rat insulin receptor will cross react. This assay is intended for detection of IR from lysates of cells.

    Analytical Method

    Quantitative

    Cross-Reactivity (Details)

    Calculated cross reactivity: Hu Mo Rt

    Sensitivity

    0.5 ng/mL

    Characteristics

    Insulin Receptor, beta, Human (IRb) ELISA Kit

    Components

    • Standard 2 x1vial
    • Standard Diluent Buffer 1x25ml
    • Microtiter Plate 1x96 wells (12 x 8 well strips)
    • IRb Pab 1x11ml. Contains 0.1% sodium azide.
    • IgG (HRP) (100x) 1x125ul. Contains 3.3mM thymol.
    • HRP Diluent. 1x25ml. ontains 3.3mM thymol.
    • Wash Buffer Concentrate (25x) 1x100ml
    • Substrate (TMB) 1x25ml
    • Stop Solution 1x25ml
  • Assay Precision

    Precision: Intra-assay:
    • <8.7%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C
  • Target See all ISR-beta ELISA Kits

    ISR-beta (Insulin Receptor beta (ISR-beta))

    Alternative Name

    Insulin Receptor, beta
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