Albumin ELISA Kit

Catalog No. ABIN5068089

Human Albumin ELISA Kit Colorimetric assay for quantification of Human Albumin.

Quick Overview for Albumin ELISA Kit (ABIN5068089)

Target: Albumin (ALB)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Sample Type: Urine

Product Details Albumin ELISA Kit

Purpose: This Micro- albumin Quantitative using microwell competitive ELISA method provides a convenient, sensitive and specific assay for albumin and free of interference from urine specimens.

Analytical Method: Quantitative

Sensitivity: 250 ng/mL

Characteristics: Albumin, Human, ELISA Kit (Serum Albumin, ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341)

Components:

• Human Albumin coated microwell strip plate: 1 X 96 wells
• Albumin Std. A: 1 X 11ml
• Albumin Std. B (2.5ug/ml): 1 X 0.5ml
• Albumin Std. C (5ug/ml): 1 X 0.5ml
• Albumin Std. D (25ug/ml): 1 X 0.5ml
• Albumin Std. E (50ug/ml): 1 X 0.5ml
• Albumin Std. F (100ug/ml): 1 X 0.5ml
• Albumin Control (exact value printed on the vial)
• Anti-Albumin-HRP Conjugate: 1 X 11ml
• HRP substrate Solution: 1 X 11ml
• Wash buffer (100X): 1 X 10ml, dilute 1:100 with distilled water
• Stop solution (ready-to-use): 1 X 10ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• Albumin ELISA kit is based on competitive binding of human albumin from urine samples and albumin coated on the microwell plate to the enzyme labeled anti- human albumin antibody. Higher concentrations of albumin in the urine samples result in decreased binding of enzyme (HRP) labeled antibody to the microwell plate. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (color) is inversely proportional to the amount of albumin present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of albumin in samples and control is read off the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Background: The analytical determination of the protein albumin in urine is important because increased values indicate an increased risk of developing end-stage renal diseases and cardiovascular disease among people with diabetes. Also albumin in urine is a sensitive indicator of renal damage caused by exposure to nephrotoxic substances. The most significant and well-documented of these abnormalities is a subtle increase in the urinary albumin excretion rate, known as mico-albuminuria. Mico-albuminuria is not measurable by conventional techniques for detecting proteinuria. It is believed that mico-albuminuria represents a reversible stage of renal dysfunction, whereas overt proteinuria reflects irreversible disease. Proteinuria typically appears about twenty years after the onset of diabetes, whereas mico-albuminuria can be detected within the first ten years. Mico-albuminuria (30-150 μg/min) has been established as a marker predictive of subsequent development of diabetic nephropathy. Periodic monitoring(2-3 times/year) of urinary of albumin levels in the diabetic patient is therefore recommended so that the initial escalation of renal damage can be detected and appropriate treatment regimens can be instituted. Radial immunodiffusion, immunoturbidimetric, immunophelometric method and RIA have been used for the albumin assay in urine.

Pathways: Lipid Metabolism