Albumin ELISA Kit

Catalog No. ABIN5068124

This Human Albumin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Albumin.

Quick Overview for Albumin ELISA Kit (ABIN5068124)

Target: Albumin (ALB)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Urine, Plasma, Media

Product Details Albumin ELISA Kit

Sensitivity: 5 ng/mL

Characteristics: Albumin, Human, ELISA Kit (Serum Albumin, ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341)

Components:

• Microtiter Plate: 1x96 wells
• Control (ng/ml), 1x650ul
• Standard (5ng/ml), 1x650ul
• Standard (10ng/ml), 1x650ul
• Standard (20ng/ml), 1x650ul
• Standard (50ng/ml), 1x650ul
• Standard (100ng/ml), 1x650ul
• HRP Conjugate, 100X, 1x150ul
• Sample Diluent, 20X, 1x10ml
• 0: Wash Solution, 100X, 1x10ml
• 1: TMB Substrate, 1x12ml
• 2: Stop Solution (1% O4), 1x12ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The Human Albumin ELISA kit is based on the binding of human albumin in samples to two antibodies, one immobilized on the microwells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate (TMB) is added and color is developed by the enzymatic reaction of HRP on the substrate, which is directly proportional to the amount of Albumin present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microwell reader. The concentration of albumin in samples and control is calculated from a curve of standards containing known concentrations of albumin.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Pathways: Lipid Metabolism