Lactoferrin ELISA Kit

Catalog No. ABIN5068137

Human Lactoferrin ELISA Kit Colorimetric assay for quantification of Human Lactoferrin.

Quick Overview for Lactoferrin ELISA Kit (ABIN5068137)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Urine, Plasma, Cell Culture Supernatant, Cerebrospinal Fluid, Bronchoalveolar Lavage

Product Details Lactoferrin ELISA Kit

Characteristics: Lactoferrin, Human Plasma ELISA Kit,

Components:

• Sample Dilution Buffer: PBS, BSA, Tween-20 and 0.01% thimerosal, 3 x 25ml
• LTF Standard: Approximately 300ng of purified LTF with BSA in lyophilized form, 1x1vial
• Wash Buffer (20x), Tris-HCl buffer containing NaCl, Tween-20, and 0.01% thimerosal. 1x100ml
• LTF Antibody, Concentrated solution of monoclonal antibody to LTF in phosphate buffer containing NaCl, BSA, glycerol, and 0.01% thimerosal. 1x75ul
• Streptavidin-HRP, Concentrated solution of streptavidin-coupled HRP in Tris-HCl buffer containing BSA and 0.01% thimerosal. 1x75ul
• Dilution Buffer, PBS, BSA, Tween-20, and 0.01% thimerosal, 3x8ml
• OPD Diluting Buffer, Citrate buffer containing 2 and 0.01% thimerosal. 1x20ml
• Stop Solution, 1M O4. 1x20ml
• OPD, Four tablets.
• Microtiter plate, 1x96 wells

Application Details

Plate: Pre-coated

Protocol: Principle of the Procedure:

• This method is an Enzyme-linked immunosorbent assay (ELISA) for lactoferrin. Lactoferrin is captured by a monoclonal antibody (MAb) that is coated on wells of a sectional microplate. A second LTF-MAb labeled with biotin is added to the well and binds with the captured LTF forming a "sandwich." A solution of streptavidin-peroxidase is then added. Streptavidin has a high affinity for biotin and once bound, its HRP (HRP) label is available for color development by addition of the substrate, o-phenylenediamine (OPD). This color development at 450nm is proportional to the quantity of LTF in the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactoferrin

Background: Human lactoferrin (LTF) is an 80kD glycoprotein which was first isolated from human milk. LTF is found in most body fluids and secretions, e.g., in the nose, genital tract, and tears.1 In the blood, LTF is secreted by neutrophils and its plasma concentration is positively related to the total pool of neutrophils and to the rate of neutrophil turnover.2 Because of its ability to strongly bind iron, LTF is considered to be bactericidal.3 In a number of cases of inflammation, LTF is released into the extracellular medium from secondary granules of neutrophilic leukocytes.4,5 Its extracellular concentration can therefore be used as an index of neutrophil activation, especially in blood samples containing anti-myeloperoxidase antibodies.

Pathways: Transition Metal Ion Homeostasis