Serotonin ELISA Kit

Catalog No. ABIN5068229

This Chemical Serotonin ELISA Kit is a Fluorometric, Colorimetric ELISA Kit designed to quantify Chemical Serotonin.

Quick Overview for Serotonin ELISA Kit (ABIN5068229)

Target: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Reactivity: Chemical

Detection Method: Fluorometric, Colorimetric

Method Type: Competition ELISA

Application: ELISA

Sample Type: Cell Culture Lysate, Plasma, Serum

Product Details Serotonin ELISA Kit

Analytical Method: Quantitative

Sensitivity: 0.08-19.8 ng/mL

Characteristics: Serotonin, Human (5-Hydroxytryptamine, 5HT, 5-HT, 5-HT5A, 5-HT-5A) ELISA Kit

Components:

• Microtiter Plate, 1x96 wells
• Serotonin Pab 1x8ml
• Serotonin (Biotin) Pab, 1x8ml
• Streptavidin (AP), 100x, 1x300ul
• Standard, 0ng/ml 1x1ml
• Standard, 0.08ng/ml 1x1ml
• Standard, 0.24ng/ml 1x1ml
• Standard, 0.73ng/ml 1x1ml
• Standard, 2.2ng/ml 1x1ml
• 0: Standard, 6.6ng/ml 1x1ml
• 1: Standard, 19.8ng/ml 1x1ml
• 2: Control 1, ng/ml, 1x500ul
• 3: Control 2, ng/ml, 1x500ul
• 4: Acylation Reagent, 1x3ml
• 5: Assay Buffer, 10x, 1x50ml
• 6: Wash Buffer, 20x, 2x50ml
• 7: Substrate (PNPP), 2x13ml
• 8: Stop Solution (1N NaOH), 1x15ml

Application Details

Protocol: Principle:

• The sample preparation (derivatization of serotonin to N-acylserotonin) is part of the sample dilution and is achieved by incubation of the respective sample with the Acylation Reagent.
• The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Serotonin

Target: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Alternative Name: Serotonin

Target Type: Chemical