Nerve Growth Factor ELISA Kit

Catalog No. ABIN5520117

Mouse Nerve Growth Factor ELISA Kit Colorimetric assay for quantification of Mouse Nerve Growth Factor and has been mentioned in 1 publication.

Quick Overview for Nerve Growth Factor ELISA Kit (ABIN5520117)

Target: Nerve Growth Factor (NGF)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details Nerve Growth Factor ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: For quantitative detection of NGF/NGFβ in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of NGF/NGFβ. No significant cross- reactivity or interference between NGF/NGFβ and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between NGF/NGFβ and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 18.75 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for Nerve Growth Factor ELISA Kit (ABIN5520117)

Foidl, Ucar, Schwarz, Rebelo, Pandit, Humpel: "Nerve growth factor released from collagen scaffolds protects axotomized cholinergic neurons of the basal nucleus of Meynert in organotypic brain slices." in: Journal of neuroscience methods, Vol. 295, pp. 77-86, (2018) (PubMed).

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: Nerve growth factor

Background: NGF/NGFβ/NGFB/beta-nerve growth factor/Beta-NGF/HSAN5/nerve growth factor(beta polypeptide)

UniProt: P01139

Pathways: Regulation of Cell Size