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IL1A ELISA Kit

This Human IL1A ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL1A.
Catalog No. ABIN5522120

Quick Overview for IL1A ELISA Kit (ABIN5522120)

Target

See all IL1A ELISA Kits
IL1A (Interleukin 1 alpha (IL1A))

Reactivity

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Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

3.906 pg/mL - 250 pg/mL

Application

ELISA

Sample Type

Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    3.906 pg/mL

    Purpose

    For quantitative detection of IL-1α in serum, plasma, tissue homogenates.

    Analytical Method

    Quantitative

    Specificity

    This assay has high sensitivity and excellent specificity for detection of IL-1α. No significant cross-reactivity or interference between IL-1α and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between IL-1α and all the analogues, therefore, cross reaction may still exist.

    Sensitivity

    2.344 pg/mL

    Components

    - ELISA Microplate
    - Lyophilized Standard
    - Sample / Standard Dilution Buffer
    - Biotin-labeled Antibody (Concentrated)
    - Antibody Dilution Buffer
    - HRP-Streptavidin Conjugate(SABC)
    - SABC Dilution Buffer
    - TMB Substrate
    - Stop Solution
    - Wash Buffer (25X)
    - Plate Sealer
    - Product Description
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    Sandwich ELISA, Double Antibody

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
    2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

    Expiry Date

    6 months
  • Target See all IL1A ELISA Kits

    IL1A (Interleukin 1 alpha (IL1A))

    Alternative Name

    IL-1alpha

    Background

    IL-1α/IL-1alpha/IL1A/IL1-A/IL1/IL1F1/Preinterleukin 1 Alpha/Hematopoietin-1/Pro-Interleukin-1-Alpha

    UniProt

    P01583

    Pathways

    NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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