SPARC ELISA Kit

Catalog No. ABIN5524715

This Human SPARC ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human SPARC.

Quick Overview for SPARC ELISA Kit (ABIN5524715)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details SPARC ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: For quantitative detection of ON in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of ON. No significant cross-reactivity or interference between ON and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between ON and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.094 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: Osteonectin

Background: ON/BM-40/Basement-membrane protein 40/BM-40/ONcysteine-rich protein/Secreted protein acidic and rich in cysteine/secreted protein, acidic, cysteine-rich(osteonectin)

UniProt: P09486

Pathways: Autophagy