Glucagon ELISA Kit

Catalog No. ABIN5526763

This Various Species Glucagon ELISA Kit is a Colorimetric ELISA Kit designed to quantify Various Species Glucagon in Plasma, Cell Culture Supernatant and Serum.

Quick Overview for Glucagon ELISA Kit (ABIN5526763)

Target: Glucagon (GCG)

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 8.42-5000 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details Glucagon ELISA Kit

Minimum Detection Limit: 8.42 pg/mL

Purpose: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of Glucagon.

Analytical Method: Quantitative

Specificity: Glucagon

Components: plate, standard, diluent

Material not included: pipettes, tubes, reader

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Buffer: 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Glucagon

Target: Glucagon (GCG)

Alternative Name: Glucagon

Background: Glucagon is a 29-amino acid polypeptide, produced by alpha cells of the pancreas. The polypeptide has a molecular weight of 3485 Da that plays a critical role in glucose metabolism and homeostasis. It works to raise the concentration of glucose in the bloodstream. It is also used as a medication to treat a number of health conditions. Its effect is opposite to that of insulin, which lowers the glucose. The pancreas releases glucagon when the concentration of glucose in the bloodstream falls too low. Glucagon causes the liver to convert stored glycogen into glucose, which is released into the bloodstream. High blood-glucose levels, on the other hand, stimulate the release of insulin. Glucagon allows glucose to be taken up and used by insulin-dependent tissues. Thus, glucagon and insulin are part of a feedback system that keeps blood glucose levels stable. Abnormally elevated levels of glucagon may be caused by pancreatic tumors, such as glucagonoma, symptoms of which include necrolytic migratory erythema, reduced amino acids, and hyperglycemia. It may occur alone or in the context of multiple endocrine neoplasia type 1.

NCBI Accession: NM_002054

UniProt: P01275

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling