PKC epsilon CLIA Kit

Catalog No. ABIN5658222

This Chemiluminescent ELISA kit is designed for the quantitative measurement of Human PKC epsilon.

Quick Overview for PKC epsilon CLIA Kit (ABIN5658222)

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Product Details PKC epsilon CLIA Kit

Minimum Detection Limit: 1.56 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase C Epsilon (PKCe). No significant cross-reactivity or interference between Protein Kinase C Epsilon (PKCe) and analogues was observed.

Sensitivity: 0.07 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Epsilon (PKCe). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protein Kinase C Epsilon (PKCe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protein Kinase C Epsilon (PKCe) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase C Epsilon (PKCe) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase C Epsilon (PKCe) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: Protein Kinase C Epsilon

Background:

Gene Name: Protein Kinase C Epsilon

Gene Aliases: PKC-E, PRKCE, PRKC-E, NPKC-Epsilon

Gene ID: 5581

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling