C9orf3 ELISA Kit

Catalog No. ABIN5690820

Human C9orf3 ELISA Kit Colorimetric assay for quantification of Human C9orf3 in Cell Culture Supernatant, Cell Samples, Plasma, Serum and Tissue Lysate

Quick Overview for C9orf3 ELISA Kit (ABIN5690820)

Target: C9orf3 (Chromosome 9 Open Reading Frame 3 (C9orf3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details C9orf3 ELISA Kit

Purpose: Human Aminopeptidase O ELISA Kit.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Aminopeptidase O.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for C9orf3

Target: C9orf3 (Chromosome 9 Open Reading Frame 3 (C9orf3))

Alternative Name: Aminopeptidase O

Gene ID: 84909

UniProt: Q8N6M6