PF4 ELISA Kit
Quick Overview for PF4 ELISA Kit (ABIN5692208)
Target
See all PF4 ELISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 156 pg/mL
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Purpose
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL4/PF4. 96wells/kit, with removable strips.
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Brand
- PicoKine™
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Analytical Method
- Quantitative
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Specificity
- Expression system for standard: E.coli, Immunogen sequence: V30-S105
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Sensitivity
- < 15 pg/mL
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Components
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96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover
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Platelet Factor 4 (PF4) ELISA Kit
PF4 Reactivity: Human Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Mouse Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Cow Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Mouse AA 30-105 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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Assay Time
- 15 min
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Expiry Date
- 12 months
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- PF4 (Platelet Factor 4 (PF4))
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Alternative Name
- Pf4
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Background
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Synonyms: Platelet factor 4, PF-4, C-X-C motif chemokine 4, Pf4, Cxcl4, Scyb4
Background: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Cellular Localisation: Secreted. -
UniProt
- P06765
Target See all PF4 ELISA Kits
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