DAND5 ELISA Kit

Catalog No. ABIN5692233

Human DAND5 ELISA Kit Colorimetric assay for quantification of Human DAND5.

Quick Overview for DAND5 ELISA Kit (ABIN5692233)

Target: DAND5 (DAN Domain Family, Member 5 (DAND5))

Binding Specificity: AA 23-189

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details DAND5 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DAND5. 96wells/kit, with removable strips.

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli, Immunogen sequence: R23-A189

Sensitivity: < 10 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for DAND5

Target: DAND5 (DAN Domain Family, Member 5 (DAND5))

Alternative Name: DAND5

Background:

Synonyms: DAN domain family member 5, Cerberus-like protein 2, Cerl-2, Cysteine knot superfamily 1, BMP antagonist 3, Gremlin-3, DAND5, CER2, CKTSF1B3, GREM3, SP1

Background: This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry.

Cellular Localisation: Secreted.

UniProt: Q8N907