RHOT1 ELISA Kit

Catalog No. ABIN5706906

This Human RHOT1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human RHOT1.

Quick Overview for RHOT1 ELISA Kit (ABIN5706906)

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details RHOT1 ELISA Kit

Minimum Detection Limit: 0.625 ng/mL

Analytical Method: Quantitative

Sensitivity: 0.272 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for RHOT1

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Alternative Name: Mitochondrial Rho GTPase 1 (RHOT1)

Background: Alternative name: Rac-GTP-binding protein-like protein, Ras homolog gene family member T1, ARHT1, MIRO1, MIRO-1