IL16 ELISA Kit

Catalog No. ABIN577083

This Human IL16 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL16.

Quick Overview for IL16 ELISA Kit (ABIN577083)

Target: IL16 (Interleukin 16 (IL16))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 20-3200 pg/mL

Application: ELISA

Product Details IL16 ELISA Kit

Minimum Detection Limit: 20 pg/mL

Purpose: This IL-16 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-16. Standards or samples are then added - the appropriate microtiter plate wells and incubated. IL-16 if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed - remove any unbound IL-16 and other components of sample. In order - quantitatively determine the amount of IL-16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for IL-16 is added - each well to

Analytical Method: Quantitative

Sensitivity: < 20 pg/mL

Components: Standards: 1 set/2 vials

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for IL16

Target: IL16 (Interleukin 16 (IL16))

Alternative Name: Interleukin-16 (IL-16)

Background: Syphilis is a complex sexually transmitted disease that is caused by the spirochaete T. pallidum. The disease evolves through primary, secondary and latent stages. Tertiary manifestations of syphilis may occur from one to thirty years after primary infection and include the development of gummatous lesions, cardiovascular syphilis and neurological syphilis. T. pallidum cannot be cultured in vitro and therefore diagnosis is dependent on clinical signs, direct observation of the bacteria from lesions, and serology. Except for very early disease, serological procedures are the preferred diagnostic method for syphilis at all stages. Two types of serological tests are used: non-treponemal and treponemal. Both non- treponemal and treponemal serology as well as clinical history is required for definitive diagnosis. Non-treponemal tests detect antibodies reactive with a lipoidal antigen consisting of cardiolipin, lecithin and cholesterol. These non-treponemal antibodies are thought to be generated in response to lipid associated with the treponeme cell surface. The non- treponemal tests may be used for screening and to monitor the effectiveness of therapy. Treponemal tests detect antibodies reactive with whole T. pallidum or sonicates thereof. Typically, sera must be adsorbed with an extract of non-pathogenic treponemes to remove cross-reactive antibodies prior to performing treponemal tests. Treponemal tests become reactive after primary infection and in most cases remain positive for the lifetime of the patient. Test sensitivity varies with the stage of syphilis (primary 69-1%, secondary 1%, latent 97-1%, and late 94-96%). False negative results are associated with early primary disease and prozone reactions can occur in the agglutination-based treponemal tests. Treponemal tests have a specificity of 94-1% in normal populations. The T. pallidum antigen has been identified as an abundant, immunodominant and putatively pathogen-specific membrane lipoprotein of T. pallidum. Antibodies to the T. pallidum antigen protein have been identified in patients with syphilis and in infants with congenital syphilis by immunoblot and by enzyme immunoassay. S7.5(2) Syphilis 2