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FADS2 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Rabbit FADS2.
Catalog No. ABIN6204443

Quick Overview for FADS2 ELISA Kit (ABIN6204443)

Target

See all FADS2 ELISA Kits
FADS2 (Fatty Acid Desaturase 2 (FADS2))

Reactivity

  • 2
  • 2
  • 1
  • 1
Rabbit

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

3.125 pg/mL - 200 pg/mL

Application

ELISA

Sample Type

Plasma, Serum
  • Minimum Detection Limit

    3.125 pg/mL

    Purpose

    Rabbit Fatty Acid Desaturase 2 (FADS2) ELISA Kit

    Analytical Method

    Quantitative

    Sensitivity

    1.88 pg/mL

    Components

    The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
    • Pre-coated 96-Well Microplate
    • Standard
    • Standard Diluent Buffer
    • Wash Buffer
    • Detection Reagent A
    • Detection Reagent B
    • Diluent A
    • Diluent B
    • TMB Substrate
    • Stop Solution
    • Plate Sealer

    Material not included

    • 37 °C incubator
    • Multi and single channel pipettes and sterile pipette tips
    • Squirt bottle or automated microplate washer
    • 1.5 mL tubes
    • Distilled water
    • Absorbent filter papers
    • 100 mL and 1 liter graduated cylinders
    • Microplate reader (wavelength: 450 nm)
    • ELISA Shaker
  • Application Notes

    Optimal dilutions/concentrations should be determined by the end user.

    Comment

    The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

    Plate

    Pre-coated

    Assay Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of FADS2 were tested 20 times on one plate, respectively.

    Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of FADS2 were tested on 3 different plates, 8 replicates in each plate.

    CV (%) = (Standard Deviation / mean) x 100

    Intra-Assay: CV<10%

    Inter-Assay: CV<10%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

    Expiry Date

    6 months
  • Target See all FADS2 ELISA Kits

    FADS2 (Fatty Acid Desaturase 2 (FADS2))

    Alternative Name

    Fatty Acid Desaturase 2
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