GOT1 ELISA Kit
Quick Overview for GOT1 ELISA Kit (ABIN6226633)
Target
See all GOT1 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 3.125 ng/mL
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Purpose
- Chicken Aspartate Aminotransferase, Cytoplasmic (GOT1) ELISA Kit
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Analytical Method
- Quantitative
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Sensitivity
- 1.88 ng/mL
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Components
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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Material not included
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA Kit
GOT1 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 Reactivity: Mouse Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 Reactivity: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 Reactivity: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitSmall Sample GOT1 Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal dilutions/concentrations should be determined by the end user.
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Comment
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The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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Plate
- Pre-coated
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Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Aspartate Aminotransferase, Cytoplasmic (GOT1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Aspartate Aminotransferase, Cytoplasmic (GOT1) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<10%
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
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Expiry Date
- 6 months
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- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
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Alternative Name
- Aspartate Aminotransferase, Cytoplasmic
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Pathways
- Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
Target See all GOT1 ELISA Kits
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