Anti-PD-1 Ab ELISA Kit
Reactivity: Mouse
Colorimetric
Competition ELISA
Serum
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- Target
- Anti-PD-1 Ab
- Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Application
- ELISA
- Purpose
- The kit has been especially developed for the quantitative analysis anti-PD-1 antibody in mouse serum samples based on the principle of competing.
- Sample Type
- Serum
- Analytical Method
- Quantitative
- Specificity
- This kit has been tested for multiple investigational PD-1 antibodies, as well as the commercially available Keytruda® and Opdivo®. There is no requirement for the species and subtype of the antibody.
- Components
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Human PD-1
Biotinylated anti-PD-1 antibody
Streptavidin-HRP
Anti-PD-1 antibody
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- Plate
- Uncoated
- Protocol
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1. Coat the plate with human PD-1
2. Add the mixture of your sample and biotinylated anti-PD-1 antibody
3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate
4. Record the OD readings and analyze the serum concentration. - Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- No activity loss was observed after storage at: -20°C for 1 year in lyophilized state after receipt, -80°C for 4 months under sterile conditions after reconstitution with carrier protein., Powder,RT
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- Target
- Anti-PD-1 Ab
- Background
- How a therapeutic antibody is metabolized in the body is pertinently relevant to its efficacy. Therefore, pharmacokinetics study is an important part of the drug development.PD-1 is probably the most targeted molecule in today's pharmaceutical industry, t
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