Thyroxine T4 ELISA Kit

Catalog No. ABIN629485

Hormone Thyroxine T4 ELISA Kit Colorimetric assay for quantification of Hormone Thyroxine T4.

Quick Overview for Thyroxine T4 ELISA Kit (ABIN629485)

Target: Thyroxine T4 (T4)

Reactivity: Hormone

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Product Details Thyroxine T4 ELISA Kit

Analytical Method: Quantitative

Characteristics: ELISA kit for detection of Thyroxine in the research laboratory
Alternative Names: Free T4 ELISA Kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Plate: Pre-coated

Assay Procedure:

The CBI fT4 is a solid phase competitive ELISA. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. fT4 in the patient's serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound fT4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT4 in the samples. A standard curve is prepared relating color intensity to the concentration of fT4.

Restrictions: For Research Use only

Handling

Precaution of Use: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Storage: 4 °C

Target Details for Thyroxine T4

Target: Thyroxine T4 (T4)

Alternative Name: Thyroxine

Target Type: Amino Acid

Background: The thyroid hormone, thyroxine (T4) is produced by the thyroid gland. An important component in the synthesis is iodine. The major form of thyroid hormone in the blood is thyroxine (T4). Thyroxine is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5'- iodinase). Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone. Only a verysmall fraction of the circulating hormone is free (unbound) - T4 0.03 %.