Prostate Specific Antigen ELISA Kit

Catalog No. ABIN629486

This ELISA kit is designed for the quantitative measurement of Human Prostate Specific Antigen.

Quick Overview for Prostate Specific Antigen ELISA Kit (ABIN629486)

Target: Prostate Specific Antigen (PSA)

Reactivity: Human

Method Type: Sandwich ELISA

Application: ELISA

Product Details Prostate Specific Antigen ELISA Kit

Characteristics: ELISA kit for detection of PSA in the research laboratory
Alternative Names: Prostate Specific Antigen ELISA Kit, Total PSA ELISA Kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

The PSA ELISA test is a solid phase two-site immunoassay. An anti-PSA monoclonal antibody is coated on the surface of the microtiter wells and a mouse anti-PSA antibody labeled with horseradish peroxidase is used as the tracer. The PSA molecules present in the standard solution or sera are 'sandwiched' between the two antibodies. Following the formation of the coated antibody-antigen-antibody-enzyme complex, the unbound proteins and antibody-enzyme tracers are removed by washing. The horseradish peroxidase activity bound in the wells is then assayed by a colorimetric reaction. The intensity of the color formed is proportional to the concentration of PSA present in the sample.

Restrictions: For Research Use only

Handling

Precaution of Use: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Storage: 4 °C

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: PSA

Background: PSA is a 32 kDa single chain glycoprotein serine protease with a chymotrypsin like specificity produced by the secretory epithelium of the prostate gland. Only low levels of PSA are normally present in the blood stream, and increasing serum concentrations indicate prostatic pathology, including benign prostatic hyperplasia and cancer of the prostate.

Pathways: Complement System