Complement C1c+C1q ELISA Kit

Catalog No. ABIN629516

Product Details

Target: Complement C1c+C1q

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for detection of Complement C1 c and C1 in the research laboratory
Alternative Names: C1c/C1q ELISA Kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Plate: Pre-coated

Assay Procedure:

Highly purified human C1q is bound to microwells. Antibodies aginst this antigen, if present in diluted serum, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Restrictions: For Research Use only

Handling

Precaution of Use: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV I/II, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Storage: 4 °C

References

McCutcheon, Quarmby, Song: "Development and optimization of a cell-based neutralizing antibody assay using a sample pre-treatment step to eliminate serum interference." in: Journal of immunological methods, Vol. 358, Issue 1-2, pp. 35-45, (2010) (PubMed).

Target Details

Target: Complement C1c+C1q

Background: The presence of Anti-C1q autoantibodies is associated with several autoimmune and renal illnesses. Containing an occurrence of 100 % in the hypocomplementaemic uricarial vasculitis syndrome (HUVS), Anti-C1q autoantibodies act as a diagnostic marker for this disease. They were also described in systemic lupus erythemathodes (SLE) and especially in lupus nephritis.