Angiotensin I Converting Enzyme 1 ELISA Kit

Catalog No. ABIN6719828

Supplier Product No.: koa0337

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Angiotensin I Converting Enzyme 1 in Cell Culture Supernatant, Plasma (heparin), Saliva and Serum.

Quick Overview for Angiotensin I Converting Enzyme 1 ELISA Kit (ABIN6719828)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Saliva, Serum

Product Details Angiotensin I Converting Enzyme 1 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0337

Supplier: Rockland

Purpose: Human ACE Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human ACE. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human ACE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum, plasma(heparin) or saliva to each empty well. We recommend that each human ACE standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: sf2NSO, Immunogen sequence: L30-L1261

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: ACE

Background:

Synonyms: ACE, ACE_HUMAN, ACE1, Angiotensin converting enzyme somatic isoform, Angiotensin I converting enzyme (peptidyl dipeptidase A) 1, Angiotensin-converting enzyme, Carboxycathepsin, CD143, CD143 antigen, DCP, DCP1, Dipeptidyl carboxypeptidase 1, Dipeptidyl carboxypeptidase I, Kininase II, MVCD3, Peptidase P, soluble form, Testicular ECA

Background: Angiotensin-converting enzyme(ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

Gene ID: 1636

NCBI Accession: NP_000780

UniProt: B4DKH4

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration