Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1) ELISA Kit

Catalog No. ABIN6719942

Supplier Product No.: koa0332

This Colorimetric ELISA kit is designed for the quantitative measurement of Human .

Quick Overview for Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1) ELISA Kit (ABIN6719942)

Target: Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Serum

Product Details

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0332

Supplier: Rockland

Purpose: Human sVEGFR1 - sFLT1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Svegfr1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 30 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 156pg/mL-10000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human sVEGFR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each human sVEGFR1 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: sf21, Immunogen sequence: S27-H687

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1)

Alternative Name: sVEGFR1/sFLT1

Background:

Synonyms: FLT, FLT 1, Flt-1, FLT1, Fms like tyrosine kinase 1, Fms related tyrosine kinase 1, Fms-like tyrosine kinase 1, Tyrosine protein kinase FRT, Tyrosine protein kinase receptor FLT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular endothelial growth factor receptor 1, Vascular endothelial growth factor/vascular permeability factor receptor, Vascular permeability factor receptor, VEGFR 1, VEGFR-1, VEGFR1, VGFR1_HUMAN

Background: sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.

Gene ID: 2321

NCBI Accession: NP_001153392

UniProt: P17948