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Thrombospondin 1 ELISA Kit

Rockland koa0540 THBS1 Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6720022
Supplier Product No.: koa0540
  • Target See all Thrombospondin 1 (THBS1) ELISA Kits
    Thrombospondin 1 (THBS1)
    Reactivity
    • 14
    • 13
    • 11
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1.56 ng/mL - 100 ng/mL
    Minimum Detection Limit
    1.56 ng/mL
    Application
    ELISA
    Supplier Product No.
    koa0540
    Supplier
    Rockland
    Purpose
    Human THBS1 - TSP1 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human THBS1. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 50 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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    Discover our top product THBS1 ELISA Kit
  • Application Notes

    ELISA_Dilution: 1.56 ng/mL-100 ng/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human THBS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human THBS1 standard solution and each sample is measured in duplicate.

    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: N19-P1170

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all Thrombospondin 1 (THBS1) ELISA Kits
    Thrombospondin 1 (THBS1)
    Alternative Name
    THBS1/TSP1 (THBS1 Products)
    Background

    Synonyms: THBS 1, THBS, THBS1, Thrombospondin 1, Thrombospondin-1, Tsp 1, TSP, Tsp1, TSP1_HUMAN

    Background: Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

    Gene ID
    7057
    NCBI Accession
    NP_003237
    UniProt
    P07996
    Pathways
    Autophagy
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