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TXN ELISA Kit

TXN Reactivity: Human Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Catalog No. ABIN6720303
  • Target See all TXN ELISA Kits
    TXN (Thioredoxin (TXN))
    Reactivity
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156 pg/mL - 10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (heparin), Serum
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: ADF, ATL-derived factor, DKFZp686B1993, MGC61975, SASP, Surface associated sulphydryl protein, Surface-associated sulphydryl protein, THIO_HUMAN, Thioredoxin, TRDX, TRX 1, Trx, TRX1, TXN, TXN protein

    Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.

    Gene Name: TXN

    Production: Natural and recombinant human Thioredoxin. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: V2-V105

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  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Thioredoxin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Thioredoxin standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 156pg/mL-10,000pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all TXN ELISA Kits
    TXN (Thioredoxin (TXN))
    Alternative Name
    Thioredoxin (TXN Products)
    Synonyms
    TRDX ELISA Kit, TRX ELISA Kit, TRX1 ELISA Kit, ADF ELISA Kit, AW550880 ELISA Kit, Trx1 ELISA Kit, Txn ELISA Kit, trx ELISA Kit, TXN ELISA Kit, SCM1.18 ELISA Kit, trxA3 ELISA Kit, thioredoxin ELISA Kit, TR-1 ELISA Kit, Trx-1 ELISA Kit, thioredoxin ELISA Kit, thioredoxin 1 ELISA Kit, thioredoxin L homeolog ELISA Kit, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) ELISA Kit, thioredoxin pseudogene ELISA Kit, cytosolic thioredoxin Trx1 ELISA Kit, TXN ELISA Kit, Txn1 ELISA Kit, txn.L ELISA Kit, txn ELISA Kit, trx ELISA Kit, SCO0885 ELISA Kit, PHG25ORF099c ELISA Kit, LOC100349046 ELISA Kit, Txn ELISA Kit, trx1 ELISA Kit
    Gene ID
    7295
    NCBI Accession
    NP_001231867
    UniProt
    P10599
    Pathways
    Carbohydrate Homeostasis, Cell RedoxHomeostasis
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