ABL1 ELISA Kit (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase)

Details for Product ABL1 ELISA Kit No. ABIN6730568
Antigen
  • ABL
  • JTK7
  • bcr/abl
  • c-ABL
  • p150
  • v-abl
  • AI325092
  • Abl
  • E430008G22Rik
  • c-Abl
  • ABL proto-oncogene 1, non-receptor tyrosine kinase
  • c-abl oncogene 1, non-receptor tyrosine kinase
  • Tyrosine-protein kinase abl-1
  • ABL1
  • Abl1
  • abl-1
  • abl1
Epitope
pTyr245
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
8
6
6
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Human Phospho-c-Abl (Tyr245) and Total c-Abl ELISA Kit. This assay semi-quantitatively measures c-Abl phosphorylated at Tyrosine-245 as well as total c-Abl in cell lysate samples.
Sample Type Cell Samples, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity This ELISA kit recognizes Human c-Abl phosphorylated at site Tyrosine-245 as well as total c-Abl.
Characteristics
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Plasmids, Primers & others Plasmids, Primers & others ABL1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name c-Abl (ABL1 ELISA Kit Abstract)
Gene ID 25
UniProt P00519
Pathways Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism
Application Notes Optimal working dilution should be determined by the investigator.
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
Supplier Images
Image no. 1 for C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit (ABIN6730568) Jurkat cells were treated with Pervanadate. Cell lysates were analyzed using this pho...
Image no. 2 for C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit (ABIN6730568) Jurkat cells were treated with Pervanadate. Solubilize cells at 4 x 10^7 cells/ml in ...
Image no. 3 for C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit (ABIN6730568) Jurkat cells were treated with Pervanadate. Cell lysates were analyzed using this pho...
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