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ABL1 ELISA Kit

ABL1 Reactivity: Human pTyr245 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Catalog No. ABIN6730567
  • Target See all ABL1 ELISA Kits
    ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
    Binding Specificity
    pTyr245
    Reactivity
    • 6
    • 4
    • 4
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-c-Abl (Tyr245) ELISA Kit. This assay semi-quantitatively measures c-Abl phosphorylated at Tyrosine-245 in cell lysate samples.
    Sample Type
    Cell Samples, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human c-Abl phosphorylated at site Tyrosine-245.
    Characteristics
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all ABL1 ELISA Kits
    ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
    Alternative Name
    c-Abl (ABL1 Products)
    Synonyms
    ABL ELISA Kit, JTK7 ELISA Kit, bcr/abl ELISA Kit, c-ABL ELISA Kit, p150 ELISA Kit, v-abl ELISA Kit, AI325092 ELISA Kit, Abl ELISA Kit, E430008G22Rik ELISA Kit, c-Abl ELISA Kit, ABL proto-oncogene 1, non-receptor tyrosine kinase ELISA Kit, c-abl oncogene 1, non-receptor tyrosine kinase ELISA Kit, Tyrosine-protein kinase abl-1 ELISA Kit, ABL1 ELISA Kit, Abl1 ELISA Kit, abl-1 ELISA Kit, abl1 ELISA Kit
    Gene ID
    25
    UniProt
    P00519
    Pathways
    Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism
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