HSD17B14 IQ-ELISA Kit

Catalog No. ABIN6951568

Human HSD17B14 ELISA Kit qPCR assay for quantification of Human HSD17B14.

Quick Overview for HSD17B14 IQ-ELISA Kit (ABIN6951568)

Target: HSD17B14 (Hydroxysteroid (17-Beta) Dehydrogenase 14 (HSD17B14))

Reactivity: Human

Detection Method: qPCR

Method Type: DNA-Binding ELISA

Detection Range: 1.22 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details HSD17B14 IQ-ELISA Kit

Minimum Detection Limit: 1.22 pg/mL

Purpose: Human Immunoquantitative (PCR-Based) HSD17B14 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Components:

• HSD17B14 Microplate (Item A): 96 well PCR plate coated with anti-Human HSD17B14
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human HSD17B14
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for HSD17B14 (Item F): 2 vials of a 4x concentrated solution of anti-Human HSD17B14 affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Material not included:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Reagent Preparation:

Recommended Dilution for serum and plasma samples2 - 20 fold

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details

Target: HSD17B14 (Hydroxysteroid (17-Beta) Dehydrogenase 14 (HSD17B14))

Alternative Name: HSD17B14

Gene ID: 51171

UniProt: Q9BPX1