Chlamydia Pneumoniae IgA ELISA Kit

Catalog No. ABIN7666548

This Colorimetric ELISA kit is designed for the quantitative measurement of Human .

Quick Overview for Chlamydia Pneumoniae IgA ELISA Kit (ABIN7666548)

Target: Chlamydia Pneumoniae IgA

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details

Purpose: anti-Chlamydia pneumoniae IgA - ELISA kit for the detection of IgA antibodies to Chlamydia pneumoniae in human serum and plasma.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips in the handling frame coated with the antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control serum, r.t.u. NC 1 vial
• 1.3 mL Positive control serum, r.t.u. PC 1 vial
• 2.0 mL Calibrator, r.t.u. 1) CAL 1 vial
• 13 mL Anti-human IgA antibodies labelled with horseradish peroxidase (anti-IgA Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Instruction manual
• Certificate
• of quality 1) r.t.u., ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-Chlamydia pneumoniae IgA is a solid-phase immunoanalytical test. The purified, homogeneous native antigen is fixed to each well of the microtiterstrips. Specific antibodies present in the patient's sample are bound during the first incubation step. After removing unbound material by washing, the presence of the specific antibodies is detected using anti-human IgA conjugate during the second incubation. The unbound peroxidase conjugate is then removed and TMB substrate is added, resulting in the development of a blue colour in positive samples. The enzyme reaction is terminated by addition of the stop solution (colour change to yellow). The intensity of the yellow colour thus developed is proportional to the concentration of antibodies in the sample. 2

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: The ELISA kit should be used within three months after opening. a. Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Under these conditions, the expiration period of the entire kit is indicated on the central label, the expiration date of the individual components is indicated on their package labels. b. Store unused strips in the sealable pouch and keep the desiccant inside. c. Kits are shipped in cooling bags, the transport time up to 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer immediately. d. Store undiluted sera in aliquots at -18 to -28 °C. Frequent freezing and thawing is not recommended. Undiluted serum samples could be stored at +2 to +10°C up to one week. e. Do not store diluted samples. Always prepare fresh.

Target Details

Target: Chlamydia Pneumoniae IgA

Target Type: Antibody