EBV EA(D) IgA ELISA Kit

Catalog No. ABIN7666557

This Colorimetric ELISA kit is designed for the quantitative measurement of Human EBV EA(D) IgA.

Quick Overview for EBV EA(D) IgA ELISA Kit (ABIN7666557)

Target: EBV EA(D) IgA (Anti-Epstein-Barr Virus Early Antigen (D) IgA (EBV EA(D) IgA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Serum

Product Details EBV EA(D) IgA ELISA Kit

Purpose: anti-EA (D) EBV IgA - EL ISA kit for detection of IgA antibodies to diffusion component of an early antigen (EA (D)) Epstein-Ba rr virus (EBV) in human sera.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips in a frame coated with the s pecific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
• 1.3 mL Positive control serum, r.t.u. CTRL + 1 vial
• 1.3 mL Negative control serum, r.t.u. CTRL - 1 vial
• 13 mL Anti-human IgA antibodies labelled with horseradish peroxidase (anti-IgA Px-conjugate), r.t.u. CONJ 1 vial
• 55 mL Wash buffer concentrate, 10x concentrate d WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Sealable pouch for unused strips Instruction manual
• Quality control certificate 1) ready to use Chromogenic substrate TMB is compatible an d interchangeable between ELISA-VIDITEST kits which contain TMB and not with other Chrom ogenic substrates TMB-O, TMB-BF. 1 http://www.vidia.cz/

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-EA (D) EBV IgA assay i s a solid-phase immunoanalytical test. The polystyrene strips are coated with a recombinant antigen that b ears immunodominant epitopes of EA protein. The anti- EA (D) antibodies, if present in the serum, bind t o the immobilized antigens. The antibodies that do not bind are washed away and those that formed compl exes with the antigens are later on recognised by animal anti-human IgA antibodies labelled with horserad ish peroxidase. The presence of labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for EBV EA(D) IgA

Target: EBV EA(D) IgA (Anti-Epstein-Barr Virus Early Antigen (D) IgA (EBV EA(D) IgA))

Alternative Name: EA-D Epstein-Barr Virus IgA

Target Type: Antibody