C6 ELISA Kit

Catalog No. ABIN7666567

This Human C6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human C6.

Quick Overview for C6 ELISA Kit (ABIN7666567)

Target: C6 (Complement Component 6 (C6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details C6 ELISA Kit

Purpose: anti-C6 - ELISA kit for detection of antibodies against C6 protein (part of VlsE) in human serum/plasma.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips coated with specific recombinant antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u.1) NC 1 vial
• 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 13 mL Anti-human animal IgM+IgG antibodies labelled with horseradish peroxidase, r.t.u (anti IgM/IgG Px-conjugate) CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate (TMB substrate), r.t.u. (TMB/H2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• .Instruction manual
• Quality control certificate 1) r.t.u. ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-C6 is a solid-phase immunoanalytical test. The polystyrene strips are coated with the specific antigens. The anti-C6 antibodies, if present in the tested sera, bind to the immobilized antigen. The bonded antibodies react later on with antibodies against human IgM and IgG labelled with horseradish peroxidase. The presence of labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for C6

Target: C6 (Complement Component 6 (C6))

Alternative Name: C6

Target Type: Antibody