NEFH ELISA Kit

Catalog No. ABIN7666568

Human NEFH ELISA Kit Colorimetric assay for quantification of Human NEFH.

Quick Overview for NEFH ELISA Kit (ABIN7666568)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cerebrospinal Fluid, Plasma, Serum

Product Details NEFH ELISA Kit

Purpose: pNF-H - ELISA kit for the quantitative determination of phosphorylated forms of heavy neurofilaments.

Brand: ELISA-VIDITEST

Analytical Method: Quantitative

Components:

• ELISA doublestrips (16-well strips) in frame with immobilised monoclonal antibody STRIPS Ab 6 strips
• 100 μL pNF-H Standard (bovine pNF-H, 1 μg/mL) STANDARD 2 vial
• s 11 mL anti pNF-H antibody (anti-pNF-H IgY) r.t.u. anti-pNF-H Ab 1 vial
• / 13 mL anti-IgY antibody conjugated to horseradish peroxidase (conjugate anti-IgY/Px) r.t.u. CONJ 1 vial
• 55 mL Wash buffer 10x concentrated WASH 10x 1 vial
• 20 mL Dilution buffer r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate (TMB) r.t.u. TMB-BF 1 vial
• 13 mL Stop solution r.t.u., STOP 1 vial
• Instruction manual
• Certificate
• of quality 1) r.t.u., ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: pNF-H is a one-step sandwich immunoassay in which the surface of the microtitre plate is coated with the mouse monoclonal antibody that specifically binds the phosphorylated forms of heavy neurofilaments NF-H. The anti-pNF-H rabbit antibodies bind to the thus-bound neurofilament of the test sample, which are subsequently visualized with horseradish peroxidase-labeled anti-IgY antibody. The amount of bound labeled antibodies is determined by color enzymatic reaction. Negative sera do not respond, slight change in the color of the wells is the background of the reaction.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: The ELISA kit should be used within three months after opening. a. Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Under these conditions, the expiration date of the kit is indicated on the ELISA kit central label, the expiration of reagents is indicated on labels on their package. b. Store unused strips in the sealable pouch and keep the desiccant inside. Store the unused strips in the sealable pouch or close them well into the zipper bag with the desiccant inside. Kits are transported in cooling bags, transport time up to 72 hours has no influence on expiration. If you find any damage at any part of the kit, please inform the manufacturer. c. Store unused test samples undiluted in aliquots and frozen at -18 to -28 °C. Repeated freezing and thawing is not recommended. If you store the serum at +2 to + 10 ° C, then use them within one week. d. Solutions of test samples r.t.u. cannot be stored. Always prepare test samples solutions r.t.u..

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: pNF-H

Target Type: Antibody