SARS-CoV-2 N-Protein IgM Antibody ELISA Kit

Catalog No. ABIN7666570

This Human ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human .

Quick Overview for SARS-CoV-2 N-Protein IgM Antibody ELISA Kit (ABIN7666570)

Target: SARS-CoV-2 N-Protein IgM Antibody

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details

Purpose: anti-SARS-CoV-2 (NP) IgM - ELISA kit for the detection of IgM antibodies to nucleocapsid protein (NP) of coronavirus SARS-CoV-2, causing the COVID-19 disease in human serum and plasma isolated from venous or capillary blood.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips in the handling frame coated with the antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control serum, r.t.u. NC 1 vial
• 1.3 mL Positive control serum, r.t.u. PC 1 vial
• 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
• 13 mL Anti-human IgM antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ 1 vial
• 2 mL RF sorbent 2), 25x concentrated RF SORB 25x 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Instruction manual
• Certificate
• of quality 1) r.t.u., ready to use 2) Goat anti-human IgG globulin

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-SARS-CoV-2 (NP) IgM is a solid-phase immunoanalytical test. The specific antigen (Nucleocapsid Protein) is fixed to each well of the microtiter strips. Specific antibodies present in the patient's sample are bound during the first incubation step. After removing unbound material by washing, the presence of the specific antibodies is detected using anti-human IgM conjugate during the second incubation. The unbound peroxidase conjugate is then removed and TMB substrate is added, resulting in the development of a blue colour in positive samples. The enzyme reaction is terminated by addition of the stop solution (colour change to yellow). The intensity of the yellow colour thus developed is proportional to the concentration of antibodies in the sample. 2

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: SARS-CoV-2 N-Protein IgM Antibody

Alternative Name: SARS-CoV-2 NP IgM

Target Type: Antibody