IgG2b ELISA Kit

Catalog No. ABIN930363

This Mouse IgG2b ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IgG2b.

Quick Overview for IgG2b ELISA Kit (ABIN930363)

Target: IgG2b

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Media, Serum

Product Details IgG2b ELISA Kit

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Mouse IgG2 b Isotyping in the research laboratory
Alternative Names: Mouse IgG2b Isotyping ELISA kit

Purification: Protein A affinity column

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Mouse IgG2b Isotyping ELISA kit

Plate: Pre-coated

Protocol: This mouse IgG Isotyping Enzyme Immunometric Assay (EIA) kit is a 2b complete kit for the determintation of mouse IgG in Tissue CultureMedia and serum. 2b Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG Standard is provided in the kit. After a simultaneous incubation with a polyclonal 2b antibody to antimouse IgG conjugated to Horseradish peroxidase, which binds to the mouse IgG 2b captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse IgG in either standards or 2b samples. - See more at: https://www.fitzgerald-fii.com/mouse-igg2b-isotyping-elisa-kit-55r-ib09601.html#sthash.TRgGsoNm.dpuf

Assay Procedure:

This mouse IgG Isotyping Enzyme Immunometric Assay (EIA) kit is a 2b complete kit for the determintation of mouse IgG in Tissue Culture Media and serum.
Please read the complete kit insert before performing this assay.
The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG Standard is provided in the kit.
After a simultaneous incubation with a polyclonal 2b antibody to antimouse IgG conjugated to Horseradish peroxidase, which binds to the mouse IgG 2b captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse IgG in either standards or 2b samples.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C

Target Details for IgG2b

Target: IgG2b

Target Type: Antibody

Background: IgG2 is divided into two subclasses, IgG2a and IgG2b. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a varying portion called the "hinge region" which is unique to each IgG. This region allows for molecular flexibility and sets IgG2a apart from its IgG counterparts. Properties of IgG2a include neutralization of toxins and diffusion in the extracellular space. Synonyms: Mouse IgG2b Isotyping ELISA kit.