Cysteinyl Leukotriene ELISA Kit
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- Target
- Cysteinyl Leukotriene
- Reactivity
- Human
- Method Type
- Competition ELISA
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of Cysteinyl Leukotriene in the research laboratory
Alternative Names: Cysteinyl Leukotriene ELISA kit
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- Application Notes
- Optimal conditions to be determined by end user
- Assay Procedure
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This Cysteinyl Leukotriene kit is a competitive immunoassay for the determination of Cysteinyl Leukotrienes in tissue culture media and urine. Please read the complete kit insert before performing this assay. The kit measures three of themajor metabolites of LTA , LTC , LTD and LTE . The kit uses a monoclonal antibody to the Cysteinyl Leukotrienes (LTC , LTD and LTE ) to bind, in a competitive manner, the Cysteinyl Leukotrienes in the sample or an alkaline phosphatase molecule which has LTC covalently attached to it. After incubation at room temperature the excess reagents are washed away and substrate is added. After a second incubation, the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405nm. The intensity of the bound yellowcolor is inversely proportional to the concentration of LTC in the standards or Cysteinyl Leukotrienes in the samples. The measured optical density of LTC is used to calculate the concentration of Cysteinyl Leukotrienes in the sample.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Cysteinyl Leukotriene
- Background
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The Cysteinyl Leukotrienes (LTC , LTD , and LTE ) are metabolites of arachidonic acid. They are formed via the 5-lipoxygenase pathway by addition of cysteine derivatives to LTA. The conversion of LTA to LTC by the enzyme LTC synthase limits the rate of Cysteinyl Leukotriene formation. The Cysteinyl Leukotrienes are potent lipid mediators in inflammation. They cause smooth muscle contractions and increased capillary permeability. Monitoring the levels of Cysteinyl Leukotrienes has been useful in several studies, such as Hepatitis B, Helicobacter pylori infections, cerebral vasospasms and bloodbrain cell contact, as well as asthma and other inflammatory responses.
Synonyms: Cysteinyl Leukotriene ELISA kit.
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