C4A ELISA Kit

Catalog No. ABIN930370

Human C4A ELISA Kit assay for quantification of Human C4A.

Quick Overview for C4A ELISA Kit (ABIN930370)

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Binding Specificity: desArg

Reactivity: Human

Method Type: Competition ELISA

Application: ELISA

Product Details C4A ELISA Kit

Characteristics: ELISA kit for the detection of Human Complement C4 a des Arg in the research laboratory
Alternative Names: Human Complement C4a des Arg ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

The IBLAmerica human Complement C4a des Arg kit is a competitive immunoassay for the determination of human C4a desArg in plasma. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to human C4a des Arg to bind, in a competitive manner, the human C4a des Arg in the standard or sample or an alkaline phosphatase molecule which has human C4a des Arg attached to it. After incubation at room temperature theexcess reagents are washed away and substrate is added. After a short incubation, the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of human C4a des Arg in either standards or samples. The measured optical density is used to calculate the concentration of human C4a des Arg.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for C4A

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Alternative Name: Complement C4a

Background: The human C4a des Arg (C4a des Arg) molecule is one of three activation fragments formed from the activation of the complement cascade. C4a des Arg is formed from C4a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of human C4a desArg was first reported in 1981 by Hugli. Human C4a des Arg contains 76 amino acids with 6 cysteines involved in disulfide bridges. The structure of C4a des Arg in human, cow, rat, and mouse is similar. C4a des Arg is a highly cationic molecule containing no carbohydrate. C4a is a potent constrictor of smooth muscle cells, and has been shown to increase vascular permeablility. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be detected specifically via inhibition of non-specific protease formation of C4a des Arg.
Synonyms: Human Complement C4a des Arg ELISA kit.

Pathways: Complement System