IgM ELISA Kit

Catalog No. ABIN930371

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IgM.

Quick Overview for IgM ELISA Kit (ABIN930371)

Target: IgM

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Media, Serum

Product Details IgM ELISA Kit

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Mouse IgM Isotyping in the research laboratory
Alternative Names: Mouse IgM Isotyping ELISA kit

Purification: Immunoaffinity chromatography.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Mouse IgM Isotyping ELISA kit

Plate: Pre-coated

Protocol: The IgM Isotyping Enzyme Immunometric Assay (EIA) kit is a complete kit for the determintation of mouse IgM in Tissue Culture Media, serum and ascites fluids. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to mouse IgM immobilized on a microtiter plate to bind the mouse IgM in the standards or sample. A mouse IgM Standard is provided in the kit. After a simultaneous incubation with a polyclonal antibody to mouse IgM conjugated to Horseradish peroxidase, which binds to the mouse IgM captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration ofmouse IgMin either standards or samples. - See more at: https://www.fitzgerald-fii.com/mouse-igm-isotyping-elisa-kit-55r-ib09609.html#sthash.l4HJvClp.dpuf

Assay Procedure:

The IBLAmerica IgM Isotyping Enzyme Immunometric Assay (EIA) kit is a complete kit for the determintation of mouse IgM in Tissue Culture Media, serum and ascites fluids.
Please read the complete kit insert before performing this assay.
The kit uses a polyclonal antibody to mouse IgM immobilized on a microtiter plate to bind the mouse IgM in the standards or sample. A mouse IgM Standard is provided in the kit. After a simultaneous incubation with a polyclonalantibody to mouse IgM conjugated to Horseradish peroxidase, which binds to the mouse IgM captured on the plate, the excess reagents are washed out and substrate is added.
After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration ofmouse IgMin either standards or samples.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C

Target Details for IgM

Target: IgM

Target Type: Antibody

Background: IgM is one of the most primitive and least specialized immunoglobulins. It is one of the five classes of antibodies found in mammals and it contains u class heavy chains. The half-life of IgM is 5 days and it has a high molecular weight of 900,000 Da, which prevents its passage into extravascular areas. IgM levels are dependent on the extent of antigenic stimulation from the environment. A developing B cell always makes IgM before any other class of antibody. IgM formed in B cells is a four-chain molecule composed of two light chains and two heavy chains. Once IgM molecules are formed in B cells, they are inserted into the plasma membrane where they act as antigen receptors. IgM is also the major antibody class involved in a primary antibody response, during which IgM molecules are secreted into the blood in the form of a pentamer. In a pentamer, five fourchain IgM units are held together by disulfide bonds and a small glycopeptide known as a J (joining) chain, giving the molecule a total of 10 antigen binding sites. Synonyms: Mouse IgM Isotyping ELISA kit.