IL-10 ELISA Kit

Catalog No. ABIN930376

Product Details IL-10 ELISA Kit

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human

Application: ELISA

Characteristics: ELISA kit for the detection of IL10 in the research laboratory
Alternative Names: Human Interleukin 10 ELISA kit, IL10 ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This human IL10 Enzyme Immunometric Assay (EIA) kit is a complete kit for the determintation of human IL10 in biological fluids. Please read the complete kit insert before performing this assay. The kit uses amonoclonal antibody to human IL10 immobilized on a microtiter plate to bind the human IL10 in the standards or sample. A recombinant human IL10 Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and abiotinylatedmonoclonal antibodyto human IL10 is added. This antibodybinds to the human IL10 captured on the plate. After a short incubation the excess antibody is washed out and Streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotinylated human IL10 antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of human IL10 in either standards or samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10 (IL10 Products)

Background: Interleukin10 (IL10) was initially identified as the 'Cytokine Synthesis Inhibitory Factor' based on the observation that mouse Th1 cytokine production was suppressed by a Th2 derived factor. IL10 is a macrophage deactivating factor, acting on macrophagemonocyte accessory cells to produce its inhibitory effects on T cells and natural killer cells. It also regulates growth and/or differentiation of B cells, mast cells, granulocytes, dendritic cells, karatinocytes and endothelial cells. The ability of IL10 to suppress Th1 activities while stimulating Th2 and humoral immune responses has been circumvented by a variety intracellular pathogens known to target macrophages. A number of parasites, bacteria, fungi and viruses depress host immune responses by either inducing host IL10 production or encode their own IL10 homologs. Besides being a potent immunosuppressant, IL10 is also an anti-pyretic and functions in mast cell homeostasis. Circulating levels of IL10 are increased in allergic asthma, systemic sclerosis, a variety of cancers, post-transplantation patients, and in sepsis. The therapeutic potential of IL10 includes rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis, and HIV infections.
Synonyms: Human Interleukin 10 ELISA kit, IL10 ELISA kit.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints