Complement C3a Des-Arg ELISA Kit
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- Target
- Complement C3a Des-Arg
- Reactivity
- Human
- Method Type
- Competition ELISA
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of Human Complement C3 a des Arg in the research laboratory
Alternative Names: Human Complement C3a des Arg ELISA kit
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- Application Notes
- Optimal conditions to be determined by end user
- Assay Procedure
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The Complement C3a des Arg (human), EIA kit is a competitive immunoassay for the determination of C3a des Arg in plasma. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to human C3a des Arg to bind, in a competitive manner, the human C3a des Arg in the sample or an alkaline phosphatase molecule which has human C3a des Arg attached to it. After incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation, the enzyme reaction is stopped and the yellow colorgenerated is read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of human C3a desArg in either standards or samples. The measured optical density is used to calculate the concentration of human C3a desArg.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Complement C3a Des-Arg
- Background
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The human C3a des Arg molecule is one of three activation fragments formed from the activation of the complement cascade. C3a des Arg is formed from C3a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of human C3a des Arg was first reported in 1975 by Hugli. Human C3a des Arg contains 77 amino acids with 6 cysteines involved in disulfide bridges between residues 2249, 2356 and 3657. The C-terminal end of C3a des Arg in human, porcine, rat, mouse and guinea pig is identical. C3a des Arg is a highly cationic molecule containing no carbohydrate. Xray crystal data shows the N and C-terminal 68 residues to have highly flexible helical structures. C3a is one of the most potent constrictors of smooth muscle cells, and guinea pig airways are hyper-responsive to C3a when pretreated with histamine. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be determined specifically via inhibition of nonspecific protease formation of C3a des Arg.
Synonyms: Human Complement C3a des Arg ELISA kit.
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