PGE2 ELISA Kit

Catalog No. ABIN930393

This Hormone PGE2 ELISA Kit is a ELISA Kit designed to quantify Hormone PGE2.

Quick Overview for PGE2 ELISA Kit (ABIN930393)

Target: PGE2 (Prostaglandin E2 (PGE2))

Reactivity: Hormone

Method Type: Competition ELISA

Application: ELISA

Product Details PGE2 ELISA Kit

Characteristics: ELISA kit for the detection of PGE2 in the research laboratory
Alternative Names: Prostaglandin E2 ELISA kit, PGE2 ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This Prostaglandin E2 (PGE2) kit is a competitive immunoassay for the quantitative determination of Prostaglandin E2 in biological fluids. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to PGE2 to bind, in a competitive manner, the PGE2 in the sample, standard, or an alkaline phosphatase molecule which has PGE2 covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405 nm. The intensity of the bound yellow color is inversely proportional to the concentration of PGE2 in either standards or samples. The measured optical density is used to calculate the concentration of PGE2.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for PGE2

Target: PGE2 (Prostaglandin E2 (PGE2))

Target Type: Hormone

Background: Prostaglandin E2 is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has been shown to have a number of biological actions, including vasodilation, both anti and proinflammatory action, modulation of sleep/wake cycles, and facilitation of the replication of human immunodeficiency virus. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.
Synonyms: Prostaglandin E2 ELISA kit, PGE2 ELISA kit.