Prostaglandin F2alpha ELISA Kit

Catalog No. ABIN930394

Hormone Prostaglandin F2alpha ELISA Kit assay for quantification of Hormone Prostaglandin F2alpha.

Quick Overview for Prostaglandin F2alpha ELISA Kit (ABIN930394)

Target: Prostaglandin F2alpha

Reactivity: Hormone

Method Type: Competition ELISA

Application: ELISA

Product Details Prostaglandin F2alpha ELISA Kit

Characteristics: ELISA kit for the detection of PGF2 alpha in the research laboratory
Alternative Names: Prostaglandin F2 ELISA kit, PGF2 ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This Prostaglandin F kit is a competitive immunoassay for the determination of Prostaglandin F in biological fluids. Please read the complete kit insert polyclonal antibody to PGF to bind, in a competitive manner, the PGF in the sample or an alkaline phosphatase molecule which has PGF covalently attached to it. After a simultaneous incubation at room temperature the excess reagents away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow coloris inversely proportional to the concentration of PGF in either standards or samples. The measured optical density is used to calculate the concentration of prostaglandin F2 alpha.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Prostaglandin F2alpha

Target: Prostaglandin F2alpha

Alternative Name: PGF2 alpha

Target Type: Hormone

Background: Prostaglandin F2 alpha (PGF2a in prostanoid nomenclature) is a naturally occurring prostaglandin used in medicine to induce labor and as an abortifacient. In domestic mammals, it is produced by the uterus when stimulated by oxytocin, in the event that there has been no implantation during the follicular phase. It acts on the corpus luteum to cause luteolysis, forming a corpus albicans and stopping the production of progesterone. Action of PGF2 alpha is dependent on the number of receptors on the corpus luteum membrane. The PGF2a isoform 8isoPGF2a was found in significantly increased amounts in patients with endometriosis, thus being a potential causative link in endometriosis-associated oxidative stress.
Synonyms: Prostaglandin F2 ELISA kit, PGF2 ELISA kit.