Cyclic GMP ELISA Kit

Catalog No. ABIN930397

Chemical Cyclic GMP ELISA Kit assay for quantification of Chemical Cyclic GMP.

Quick Overview for Cyclic GMP ELISA Kit (ABIN930397)

Target: Cyclic GMP (cGMP)

Reactivity: Chemical

Application: ELISA

Product Details Cyclic GMP ELISA Kit

Characteristics: ELISA kit for the detection of cGMP in the research laboratory
Alternative Names: cGMP ELISA kit, Cyclic Guanosine monophosphate ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This cyclic GMP Complete Enzyme Immunometric Assay (EIA) kit is a kit for the determination of cyclic GMP in cells and tissue treated with 0.1N HCl, in addition to culture supernates, saliva, and serum. The optional acetylated assay format provides an approximate 10fold increase in sensitivity and is ideal for samples with extremely low levels of cGMP. If expected levels of cGMP are unknown, the investigator may evaluate a few samples in the nonacetylated format in order to determine if higher sensitivity is required.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Cyclic GMP

Target: Cyclic GMP (cGMP)

Alternative Name: cGMP

Target Type: Chemical

Background: Guanosine 3',5'cyclic monophosphate (cyclic GMP, cGMP) was identified in 1963. It has been shown to be present at levels typically 10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. It is involved in a number of important biological reactions. Some hormones, such as acetylcholine, insulin, and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators niroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels. Peptides, such as atrial natriuretic peptide (ANP), that relax smooth muscle also increase cGMP concentrations5. cGMP has been confirmed as a second messenger for ANP. NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells.
Synonyms: cGMP ELISA kit, Cyclic Guanosine monophosphate ELISA kit.