LGALS3BP ELISA Kit

Catalog No. ABIN930449

This Colorimetric ELISA kit is designed for the quantitative measurement of Human LGALS3BP.

Quick Overview for LGALS3BP ELISA Kit (ABIN930449)

Target: LGALS3BP (Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details LGALS3BP ELISA Kit

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Mac2 BP in the research laboratory
Alternative Names: Mac2BP ELISA kit, Mac-2-Binding Protein ELISA kit, L3 antigen ELISA kit, Basement membrane Autoantigen p105 ELISA kit, Serum protein 90K ELISA kit, M2BP ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

An anti90K/Mac2 BP monoclonal coating antibody is adsorbed onto microwells. 90K/Mac2 BP present in the sample orstandard binds to the antibody adsorbed to the microwells. Following incubation a HRPconjugated monoclonal anti90K/Mac2 BP antibody is added and binds to 90K/Mac2 BP captured by the first antibody. Unbound enzyme conjugated anti90K/Mac2 BP is removed during a wash step and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of soluble 90K/Mac2 BP present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from five 90K/Mac2 BP standards and 90K/Mac2 BP sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for LGALS3BP

Target: LGALS3BP (Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP))

Alternative Name: Mac2BP

Background: 90K/Mac2 BP is a widely expressed secreted 90 kDa human serum glycoprotein, originally identified in the supernatant of human breast cancer cells. It is identical to Mac2 BP, the ligand for the secreted lactose/galactose specific S-lectin Mac2 which is highly expressed by cells of the macrophage/monocyte lineage as well as a variety of other cell types. Although the biological functions and possible roles of 90K/Mac2 BP are largely unknown, some evidence indicates a role in the immunitary defence mechanism. Serum levels of 90K/Mac2 BP have been determined in patients with various forms of neoplasia and in some viral infections. In breast and colorectal cancer and in non-Hodgkin lymphoma, high levels of 90K/Mac2 BP are correlated with a poor prognosis. In HIV infection, high serum concentrations of 90K/Mac2 BP may serve as a predictor of faster progression to AIDS, independently of the numbers of CD4+ lymphocytes. In a series of HCV-infected patients, a correlation was found between elevated serum levels of 90K/Mac2 BP and failure to respond to treatment with alpha interferon. The determination of 90K/Mac2 BP in the serum can therefore be used as a valuable parameter for monitoring the outcome of cancer and viral infections.
Synonyms: Mac2BP ELISA kit, Mac-2-Binding Protein ELISA kit, L3 antigen ELISA kit, Basement membrane Autoantigen p105 ELISA kit, Serum protein 90K ELISA kit, M2BP ELISA kit.