Corticosterone ELISA Kit
Quick Overview for Corticosterone ELISA Kit (ABIN930554)
Target
See all Corticosterone (CORT) ELISA KitsReactivity
Method Type
Application
-
-
Characteristics
-
ELISA kit for the detection of Corticosterone in the research laboratory
Alternative Names: Corticosterone ELISA kit
-
-
-
-
Application Notes
- Optimal conditions to be determined by end user
-
Assay Procedure
-
This Corticosterone ELISA Kit is a solid phase enzymelinked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the Corticosterone molecule. Endogenous Corticosterone of a patient sample competes with a Corticosterone horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugateis washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Corticosterone in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Corticosterone in the patient sample.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- Store at 2-8 °C.
-
-
- Corticosterone (CORT)
-
Alternative Name
- Corticosterone
-
Target Type
- Hormone
-
Background
-
Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone is a major indicator of stress since stress increases the production of corticosteroids. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval, chronic corticosterone elevation due to dietary restrictions and in response to burn injuries. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.
Synonyms: Corticosterone ELISA kit.
Target See all Corticosterone (CORT) ELISA Kits
-