Osteocalcin ELISA Kit

Catalog No. ABIN930560

Product Details Osteocalcin ELISA Kit

Target: Osteocalcin (BGLAP)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Osteocalcin in the research laboratory
Alternative Names: Osteocalcin ELISA kit

Purification: Purified human PBL T cells

Application Details

Application Notes: Optimal conditions to be determined by end user

Plate: Pre-coated

Assay Procedure:

This osteocalcin assay is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiter plates. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of human osteocalcin. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1-human osteocalcin-MAb 2-HRP, the microtiter plate is washed to remove unbound enzyme labelled antibody. Bound enzymelabelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiter plate is then read at the appropriate wavelength. The amount of substrate turnover is determined colorimetrically by measuring the absorbance, which is proportional to the osteocalcin concentration. A calibration curve is plotted and OST concentration in samples is determined by interpolation from the calibration curve. The use of the EASIA reader (linearity up to 3 OD units) and a sophisticated data reduction method (polychromatic data reduction) result in a high sensitivity in the low range and in an extended calibration range.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Osteocalcin

Target: Osteocalcin (BGLAP)

Alternative Name: Osteocalcin (BGLAP Products)

Background: Osteocalcin or bone Gla protein (B.G.P) is the major non-collagen protein of the bone matrix. It has a molecular weight of 5800 Da and contains 49 amino acids, including 3 residues of gamma carboxyl glutamic acid. Osteocalcin is synthesized in the bone by the osteoblasts. After production, it is partly incorporated in the bone matrix and the rest is found in the blood circulation. The exact physiological function of osteocalcin is still unclear. A large number of studies show that the circulating levels of osteocalcin reflect the rate of bone formation.
Synonyms: human Osteocalcin ELISA kit.