Calcitonin ELISA Kit

Catalog No. ABIN930561

This Human Calcitonin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Calcitonin.

Quick Overview for Calcitonin ELISA Kit (ABIN930561)

Target: Calcitonin (Calca)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details Calcitonin ELISA Kit

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Calcitonin in the research laboratory
Alternative Names: Calcitonin ELISA kit, CT ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Plate: Pre-coated

Assay Procedure:

This Calcitonin ultra sensitive ELISA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiter plate. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 - human CT - MAb 2 - HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzymelabelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of stop solution and the microtiter plate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the Calcitonin concentration.A calibration curve is plotted and Calcitonin concentration in samples is determined by interpolation from the calibration curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Calcitonin

Target: Calcitonin (Calca)

Alternative Name: Calcitonin

Background: Calcitonin (CT) is a 32 amino acid peptide hormone secreted by the parafollicular C cells of the thyroid gland under serum calcium control. After acute administration, this peptide acts as a potent hypocalcemic and hypophosphatemic hormone by increasing renal calcium clearance and reducing bone resorption. However, its precise physiological role in bone metabolism is not yet fully understood. Various forms of CT may be detected in blood samples, including a CT monomer, an oxidized monomer, a dimer, higher molecular weight forms, and possibly precursor of CT. The concentrations of these peptides vary with clinical status, renal function and tissular origin of CT (normal or ectopic production). Medullary thyroid carcinoma (MTC) is a malignant tumor, developed from the C cells, secreting calcitonin in large excess. This disease occurs either as a sporadic (80 %) or a familial (20 %) form, which is transmitted as an autosomal dominant gene or as a component of multiple endocrine neoplasia (IIb). Moderate hypercalcitoninemia is also observed in pregnancy, pernicious anaemia, renal failure and during the neonatal period. Preferably, monomer form of CT is detected in this assay.
Synonyms: Calcitonin ELISA kit, CT ELISA kit.