Anti-Parvovirus IgG ELISA Kit
Quick Overview for Anti-Parvovirus IgG ELISA Kit (ABIN930637)
Target
Reactivity
Application
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Characteristics
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ELISA kit for the detection of Parvovirus IgG in the research laboratory
Alternative Names: Parvovirus IgG ELISA kit -
Purification
- Serum
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Application Notes
- Optimal conditions to be determined by end user
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Assay Procedure
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This Parvovirus B19 IgG (Recombinant) ELISA Kit is a solid phase enzymelinked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with recombinant Parvovirus B19 antigen (VP1 proteins). Diluted patient specimens and readyforuse controls are pipetted into these wells. During incubation Parvovirus B19specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman IgG antibodies are dispensed into the wells. During a second incubation this antiIgG conjugate binds specificly to IgG antibodies resulting in the formation of enzymelinked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Parvovirus B19specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store at 2-8 °C.
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- Anti-Parvovirus IgG
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Target Type
- Antibody
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Background
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The B19 virus, generally referred to as parvovirus B19 or sometimes erythrovirus B19, was the first (and until 2005 the only) known human virus in the family of parvoviruses, genus erythrovirus. B19 virus causes a childhood rash called fifth disease or erythema infectiosum which is commonly called slapped cheek syndrome. The virus was discovered by chance in 1975 by Australian virologist Yvonne Cossart. It gained its name because it was discovered in well B19 of a large series of petri dishes labelled in this way. .
Synonyms: Parvovirus IgG ELISA kit.
Target
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