Dopamine ELISA Kit

Catalog No. ABIN930670

Chemical Dopamine ELISA Kit assay for quantification of Chemical Dopamine.

Quick Overview for Dopamine ELISA Kit (ABIN930670)

Target: Dopamine (DA)

Reactivity: Chemical

Method Type: Competition ELISA

Application: ELISA

Product Details Dopamine ELISA Kit

Characteristics: ELISA kit for the detection of Dopamine in the research laboratory
Alternative Names: Dopamine ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Assay Procedure:

Dopamine is extracted by using a cisdiolspecific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Dopamine

Target: Dopamine (DA)

Alternative Name: Dopamine

Target Type: Chemical

Background: Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptor (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses.
Synonyms: Dopamine ELISA kit.