Thyroglobulin ELISA Kit

Catalog No. ABIN930684

This Hormone Thyroglobulin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Hormone Thyroglobulin.

Quick Overview for Thyroglobulin ELISA Kit (ABIN930684)

Target: Thyroglobulin (TG)

Reactivity: Hormone

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Product Details Thyroglobulin ELISA Kit

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Thyroglobulin in the research laboratory
Alternative Names: Thyroglobulin ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

Highly purified human thyroglobulin (TG) is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Alternative Name: Thyroglobulin

Target Type: Hormone

Background: Thyroglobulin (hTg), a water soluble glycoprotein with a molecular weight of approx. 660,000 Da, is the principal constituent of the thyroidal colloid sharing about 75 % of it's mass. Synthesis of the thyroid hormones T3 and T4 is based on the oxidative iodination of thyrosine residues of the thyroglobulin molecule. Within the cell thyroglobulin is transported by the microsomes. Together with the secretion of T3 and T4 also small amounts of hTg are liberated into circulation.
Synonyms: Thyroglobulin ELISA kit.

Pathways: Thyroid Hormone Synthesis