Histone ELISA Kit
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- Target
- Histone
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Reactivity
- Various Species
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
- Characteristics
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ELISA kit for the detection of Histone in the research laboratory
Alternative Names: Histone ELISA kit
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Histone ELISA Kit
Reactivity: Dog Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- Application Notes
- Optimal conditions to be determined by end-user
- Plate
- Pre-coated
- Assay Procedure
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Highly purified total histones are bound to microwells. Antibodies to these antigens, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Histone
- Background
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All of the nucleic acids (DNA) of eukaryotic cells are associated with proteins. The complex structure of DNA and its associated small basic proteins, which are called histones is known as chromatin. The histones comprise about 50 percent of the total mass of eukaryotic chromosomes. The complex of DNA and the histones can be dissociated by treatment of the eukaryotic chromatin with salt or diluted acids. Five different types of histones are known. The are called H1, H2A, H2B, H3 and H4. Their molecular weights range from 11 to 21 kDa. In correlation to their fundamental function in the organisation of chromatin, the structure of all histones in all eukaryotes is highly conserved. They contain a lot of basic amino acid residues which presumably interact with the negatively charged groups of DNA. Furthermore they contain apolar amino acid residues which may be important for their interaction among each other.
Synonyms: Histone ELISA kit.
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